|
KMID : 0895820210310040001
|
|
Journal of Oriental Rehabilitation Medicine
2021 Volume.31 No. 4 p.1 ~ p.11
|
|
|
Anti-neuroinflammatory Effects of Hwanggeumjakyak-tang on Lipopolysaccharide-induced Brain Injury Model in vivo and in vitro
|
|
Kim Jong-Gyu
Im Ji-Sung
An Sung-Hu
Song Yung-Sun
|
|
|
Abstract
|
|
|
Objectives : Hwanggeumjakyak-tang (HJT) has traditionally been used to treat gastrointestinal inflammatory diseases; however, its protective effects against neuronal inflammation are still undiscovered.
Methods : We investigated the anti-neuroinflammatory effects of HJT water extract on lipopolysaccharide (LPS)-stimulated BV2 mouse microglia cells. BV2 cells were treated with LPS (1 μg/mL) 1 hour prior to the addition of HJT. We measured cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and nitrite production using the Griess assay. We performed a reverse transcriptionpolymerase chain reaction assay to measure messenger RNA expression of inflammatory cytokines including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Western blot analysis was performed to determine protein expression of mitogen-activated protein kinases (MAPKs) and inhibitor of nuclear factor kappa B (NF-κB)α.
Results : HJT inhibited excessive nitrite release in LPS-stimulated BV2 cells and also significantly inhibited inflammatory cytokines such as IL-1β, IL-6, and TNF-α in LPS-stimulated BV2 cells. Moreover, HJT significantly suppressed LPS-induced MAPK and NF-κB activation and inhibited the elevation of IL-1β, IL-6, and TNF-α in the brain of LPS-injected mice.
Conclusions : Our study highlights the anti-neuroinflammatory effects of HJT via MAPK and NF-κB deactivation.
|
|
|
KEYWORD
|
|
|
Hwanggeumjakyak-tang (HJT), Anti-neuroinflammatory effect, Neuroprotective agents, Pro-inflammatory cytokines Microglia cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|